Electroporation is a technique used to introduce macromolecules, such as DNA and RNA, into cells by applying short electrical pulses.
Cell membranes are destroyed when cells are subjected to high voltages. However, if a critical voltage is applied for a very short duration, transient pores form in the cell membrane, which then spontaneously reseal. At this time, substances can be exchanged between the inside and outside of the cell.
Electroporation is used as a method of transfection where a short pulse of electric current is applied to perforate cells, and nucleic acids are introduced into the cells.
This method enables efficient gene transfection. Therefore, electroporation referred to as electropermeabilization is used in medical applications. It can be employed to effectively introduce genes into blood cells and lymphocytes, which was difficult with conventional methods.
Furthermore, the equipment is user-friendly and does not require specialized skills, allowing for highly reproducible experimental results.
Matsusada Precision's high voltage power supplies and amplifiers are available for pulsing target cells in electroporation.
- Related words:
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- Pulse
- Cell
- Perforation
- Cell Separation
- Flow cytometry
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Matsusada Precision provides high voltage power supplies and amplifiers for pulsing target cells in electroporation.
